tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing

tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing

Blog Article

Abstract Recent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis.Here, we present tappAS, Pump the first framework to enable a comprehensive Functional Iso-Transcriptomics (FIT) analysis, which is effective at revealing the functional impact of context-specific post-transcriptional regulation.tappAS uses isoform-resolved annotation of coding and non-coding functional domains, motifs, and sites, in combination with novel analysis methods to interrogate different aspects of the functional readout of Drive Motor W/ Flywheel transcript variants and isoform regulation.tappAS software and documentation are available at https://app.

tappas.org.